Composite

Part:BBa_K1867024

Designed by: Shien Yang Lee   Group: iGEM15_NYU-AD   (2015-09-16)


pro_TnaA_TnaB_term

TryptophanaseA and TryptophanaseB coding sequences under control of single constitutive promoter and RBS. Followed by double terminator. Used to increase production of indole in E. coli by increasing rate of tryptophan uptake and degradation via pyruvate pathway.

Contribution - Part Characterization

Group: NYU-AD 2016

Author: Sannia Farrukh

Summary: The NYUAD 2016 iGEM Team has validated the production of Indole in E. coli cells transformed with Part:BBa_K1867024 using the Kovac’s reagent tube test. Figure below represents the change in color as an indication of indole production from transformed cells. The test was done according to the manufacturer's protocol which can be found here


"T--NYU-AD--figure-4.png"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


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